An asian palm civet in the forest at night. The civet is a cat-sized mammal with grey and spotted fur and a mask-like pointed face and rounded ears.

Source: © Chuchart Duangdaw/Getty Images

Kopi luwak is made by harvesting beans that have been eaten and then excreted by Asian palm civets. The practice of harvesting beans from civet faeces is thought to have begun in the 18th century when local workers in Indonesia, who were forbidden from harvesting coffee beans by Dutch colonial authorities, discovered that the beans eaten by civets passed through the animals undamaged

Coffee beans that have been eaten and passed through the Asian palm civet have a higher concentration of fat and compounds known for their flavour-enhancing and dairy-like properties, which could explain their superior taste.

Civet coffee, also known as kopi luwak, is produced after the Asian palm civet feeds on ripened coffee berries. When the pulp is digested, the beans are excreted and these samples are collected and processed further to recover the coffee beans, which then go on to be roasted.

At $600–$1300 (£450–£975) per pound of beans, it is one of the most expensive varieties of coffee and is said to have an enhanced aroma, flavour and nutritional value. There is debate on the ethical issues of civet farming for coffee production, however in this study the civets were wild.

Animal droppings on the forest floor. The shapes of pale coffee beans are clearly visible in the droppings.

Source: © Ramit Mitra

Faeces, also known as a scat, of the Asian common palm civet, before collection by the researchers.

The study set out to compare the physical and chemical characteristics of civet-derived and manually harvested Robusta coffee beans from coffee estates across Kodagu in the Western Ghats biodiversity hotspot in south India.

The coffee beans used in the study were unroasted to preserve their native chemical profiles and were ground to produce a fine powder for chemical testing. Using gas chromatography–mass spectrometry they carried out profiling of the volatile and semi-volatile compounds and fatty acid methyl esters.

The scientists found that the civet coffee beans had a significantly higher fat content with elevated levels of caprylic acid and capric acid methyl esters – compounds known for their flavour-enhancing properties and dairy-like aroma. They added that it was likely that the natural fermentation involved in the digestive process of the civet modified the chemical composition of the beans, intensifying their flavour and giving the coffee ‘distinctive sensory characteristics’.

‘The chemical comparison further supports the notion that civet coffee is chemically different from conventionally produced coffee of similar types, mainly due to fermentation,’ they concluded.

They added that in future it would be valuable to expand testing to include Arabica varieties of coffee beans, from which most civet coffee is made, and investigate the impact of roasting on the chemical composition.