UV can introduce oxidation artefacts during protein purity tests
HPLC–UV–MS blamed for impurities spotted by AstraZeneca team
A technique routinely used to analyse proteins has been found to be introducing artefacts. The scientists at UK drug giant AstraZeneca discovered that high performance liquid chromatography (HPLC) combined with UV and mass spectroscopy (MS), which is used to assess the purity of active drug compounds like proteins or oligonucleotides, was causing oxidation impurities.